Translational_Unit

Part:BBa_K567011:Design

Designed by: Bin Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)


PT7-TDRS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal NheI site found at 1556
    Illegal PstI site found at 1152
    Illegal NotI site found at 1620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal BamHI site found at 1589
    Illegal XhoI site found at 1629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS.


Source

aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli

References